| 2.2. Rearing medaka | |
| JST Kondoh Protocols | (Appendix 2-1) |
| 2.3. Feeding | |
| Protocol for Artemia preparation | (Appendix 2-2) |
|
Column 6.2. Easy method for preparation of a small amount of hatching enzyme solution |
(Appendix 6-2) |
| 7.1. Microinjection technique for medaka eggs | |
| Detailed description of microinjection | (Appendix 7-1) |
| Microinjection using upright microscope | (Appendix 7-2) |
| 9.1. Medaka genome project and genome sequence database |
|
| Fosmid library construction protocol | (Appendix 9-1) |
| 10.1. Cell culture from medaka embryo | |
| Protocol for cell culture | (Appendix 10-1) |
| 10.7. Mutagenesis | |
| Protocol for mutagenesis | (Appendix 10-2) |