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Chapter 2
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M2-1
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Water flow into tanks
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Medaka do better with a slow water flow in drops.
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Producer: ODA
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Time: 0'20
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Filesize: 15.4 MB
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M2-2
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Washing the filtering materials
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Stir the filtering bed with hands to clean it (once a month).
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Producer: ODA
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Time: 0'25
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Filesize: 22.3 MB
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M2-3
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Washing the filtering mat
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Remove the plastic pad and wash it with running tap water (every 2–4 weeks).
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Producer: ODA
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Time: 0'52
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Filesize: 45.5 MB
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M2-4
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Cleaning the tanks
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Polish the tank walls periodically with a small piece of filtering pad to remove algae and to obtain clear view of fishes. |
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Producer: ODA
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Time: 1'11
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Filesize: 62.2 MB
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M2-5
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Removal of bottom debris I
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Debris and algal mass at the bottom of the tanks can be removed using a handmade vacuum-cleaning device.
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Producer: ODA
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Time: 0'40
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Filesize: 28.6 MB
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M2-6
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Removal of bottom debris II
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Bottom debris can be removed manually with a pipette.
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Producer: ODA
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Time: 0'35
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Filesize: 29.5 MB
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M2-7
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Removal of surface scum
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White bacterial scum forming on the water surface without water flow must be removed using a sheet of Kimwipe before feeding every morning.
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Producer: ODA
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Time: 0'19
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Filesize: 16.6 MB
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Chapter 3
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M3-1a
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Mating behavior of medaka
(Failure to spawn)
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A male fish attempts to mate a female but fails.
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Producer: KAMEI
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Time: 0'30
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Filesize: 18.8 MB
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M3-1b
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Mating behavior of medaka
(Successful spawning)
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The male is finally accepted by the female, and she spawns about 20 eggs.
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Producer: KAMEI
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Time: 1'09
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Filesize: 45.3 MB
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M3-2.
Methods of egg collection and related procedures.
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M3-2a
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Egg collection with a fish net
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Fertilized eggs are collected from a female using a fish net.
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Producer: KAMEI
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Time: 0'39
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Filesize: 40.6 MB
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M3-2b
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Transfer of the eggs into a dish
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Collected eggs are handled with tweezers and are transferred into culture medium.
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Producer: KAMEI
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Time: 0'26
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Filesize: 28.2 MB
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M3-2c
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Egg collection with a pipette
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An alternative method using a pipette is less stressful for female fish, but more difficult. |
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Producer: KINOSHITA
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Time: 0'27
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Filesize: 16.8 MB
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M3-2d
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Separation mating for scheduled spawning
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The spawning time can be controlled by separating the male and female with a thin plastic plate.
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Producer: KINOSHITA
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Time: 0'47
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Filesize: 35.4 MB
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M3-3.
Methods of egg separation
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M3-3a
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Egg separation with fingers
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Attaching filaments can be removed by pressing and rotating the egg cluster on a Petri dish with fingers.
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Producer: KAMEI
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Time: 0'34
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Filesize: 24.4 MB
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M3-3b
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Egg separation with tweezers I
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Attaching filaments can be removed with tweezers. The microscopic view is shown in M3-3c.
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Producer: KAMEI
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Time: 0'36
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Filesize: 25.4 MB
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M3-3c
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Egg separation with tweezers II
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This method is easy and quick for separating a large number of eggs (Microscopic view of M3-3b).
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Producer: KINOSHITA
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Time: 0'34
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Filesize: 26.8 MB
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M3-3d
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Egg separation with scissors
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Microscopic view of removal of attaching filaments with scissors. This method can be applied to a small number of eggs.
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Producer: KINOSHITA
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Time: 0'32
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Filesize: 19.7 MB
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M3-4
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Two methods of gathering eggs to the center of a Petri dish
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To examine eggs in a Petri dish under a stereomicroscope, the eggs can be gathered by rotating the dish or by stirring with a transfer pipette.
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Producer: KAMEI
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Time: 0'26
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Filesize: 19.8 MB
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Chapter 4
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M4-1
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Pasteurization of the eggs with bleaching solution
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When you receive eggs from other laboratories, it is necessary to pasteurize the eggs to prevent infectious diseases.
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Producer: KAMEI
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Time: 1'29
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Filesize: 84 MB
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M4-2a
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Sperm freezing
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Procedures for cryopreservation of medaka sperm are presented.
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Producer: SASADO
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Time: 3'12
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Filesize: 185 MB
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M4-2b
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Sperm motility I
(non frozen sperm)
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The motility and density of sperm are checked in each sperm suspension under a phase contrast microscope before freezing.
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Producer: SASADO
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Time: 0'04
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Filesize: 18.8 MB
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M4-3.
Methods of artificial insemination and related procedures
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M4-3a
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Collection of unfertilized eggs
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Unfertilized eggs can be collected from an anaesthetized female by squeezing her abdomen
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Producer: KAMEI
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Time: 0'47
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Filesize: 38.9 MB
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M4-3b
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Selection of eggs for artificial insemination
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Non-activated eggs suitable for artificial insemination can be clearly distinguished from “activated eggs.”
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Producer: KAMEI
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Time: 1'09
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Filesize: 66.3 MB
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M4-3c
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Sperm-thawing steps of artificial insemination
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Procedures for thawing of medaka sperm are presented.
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Producer: SASADO
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Time: 1'39
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Filesize: 81.3 MB
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M4-3d
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Insemination of unfertilized eggs with thawed sperm
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Procedures for insemination of unfertilized eggs with thawed sperm are presented. |
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Producer: KAMEI
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Time: 1'37
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Filesize: 129 MB
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M4-3e
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Motility of thawed sperm
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The motility and density of sperm are checked immediately after insemination.
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Producer: SASADO
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Time: 0'04
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Filesize: 30.5 MB
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M4-4
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Infertile mating
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The infertile mating method is highly practical to collect unfertilized eggs for artificial insemination.
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Producer: KAMEI
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Time: 1'12
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Filesize: 76.7 MB
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Chapter 6
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M6-1
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Embryonic development from fertilization to hatching
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This movie shows embryonic development from fertilization to hatching.
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Producer: JST ERATO/SORST Kondo project
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Time: 1'28
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Filesize: 48.0 MB
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M6-2.
Heart beat of developing embryos (stages 24 to 36)
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M6-2a
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Heart development I
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Stage 24, right lateral view. The heart tube has initiated a rhythmic beat by this stage. |
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Producer: MURATA
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Time: 0'15
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Filesize: 10.4 MB
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M6-2b
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Heart development II
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Stage 24–25, left lateral view. Heart tube development continues.
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Producer: MURATA
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Time: 0'15
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Filesize: 11.3 MB
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M6-2c
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Heart development III
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Stage 25, right lateral view.
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Producer: MURATA
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Time: 0'15
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Filesize: 10.1 MB
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M6-2d
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Heart development IV
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Stage 26, ventral view. |
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Producer: MURATA
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Time: 0'15
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Filesize: 7.66 MB
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M6-2e
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Heart development V
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Stage 28, right lateral view. |
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Producer: MURATA
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Time: 0'15
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Filesize: 11.9 MB
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M6-2f
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Heart development VI
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Stage 28–29, ventral view. |
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Producer: MURATA
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Time: 0'15
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Filesize: 7.46 MB
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M6-2g
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Heart development VII
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Stage 35–36, head and beating heart. |
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Producer: MURATA
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Time: 0'15
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Filesize: 12.3 MB
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M6-3
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Removing short villi on the egg envelope
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To obtain a clear view, remove short villi from the surface of egg envelope by rolling on a fine sandpaper.
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Producer: KINOSHITA
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Time: 1'05
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Filesize: 60.6 MB
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Chapter 7
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M7-1.
Methods of microinjection and related procedures
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M7-1a
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Microinjection using an upright microscope
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This movie shows the flow of microinjection using an upright microscope.
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Producer: KINOSHITA
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Time: 2'19
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Filesize: 156 MB
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M7-1b
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Separation mating for scheduled spawning
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To inject into 1-cell stage embryo, fertilized eggs must be collected
and injected within a half hour after mating. Separation mating method
is highly practical to control the time of spawning.
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Producer: KINOSHITA
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Time: 0'47
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Filesize: 35.4 MB
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M7-1c
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Preparation of eggs for microinjection
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Long attaching filaments should be removed before microinjection.
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Producer: KINOSHITA
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Time: 0'30
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Filesize: 22.7 MB
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M7-1d
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Adjusting the egg orientation for microinjection
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Prior to microinjection, the cytoplasm is oriented to where the needle will arrive, and the egg should be fixed on the egg holder.
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Producer: KINOSHITA
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Time: 0'47
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Filesize: 42.7 MB
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M7-1e
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Opening the tip of the microinjection needle
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It is necessary to break and open the tip of microinjection needle before use.
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Producer: KINOSHITA
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Time: 0'22
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Filesize: 16.1 MB
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M7-1f
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Microinjection into 1-cell cytoplasm
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This movie shows injection of solution into 1-cell cytoplasm.
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Producer: KINOSHITA
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Time: 0'37
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Filesize: 43.3 MB
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M7-2
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Making needle using a needle puller
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Horizontal and vertical type of pullers can be used to make injection needles.
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Producer: KINOSHITA
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Time: 1'09
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Filesize: 53.4 MB
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M7-3
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Sharpening the end of injection needle
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Needle with a sharpened tip is sometimes preferable.
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Producer: KINOSHITA
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Time: 1'23
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Filesize: 69.7 MB
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Chapter 10
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M10-1
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Single cell labeling
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Movement of individual cells in an early embryo is shown. The cells move, divide, and form neural compartments during neurogenesis.
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Producer: HIROSE
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Time: 0'42
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Filesize: 11.8 MB
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M10-2a
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Imaging for living embryo I
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Time-lapse imaging of the medial migration of primordial germ cells at stages 16–17.
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Producer: SAITO
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Time: 0'14
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Filesize: 12.8 MB
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M10-2b
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Imaging of living embryo II
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Time-lapse imaging of the posterior migration of primordial germ cells at stages 23–24.
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Producer: SAITO
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Time: 0'11
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Filesize: 9.34 MB
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M10-3
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ENU Mutagenesis
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This movie shows the flow of ENU mutagenesis.
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Producer: JST ERATO/SORST Kondo project
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Time: 2'02
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Filesize: 35.6 MB
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