| Contents | |
|---|---|
| 10.1. Cell culture from medaka embryo | (Figure 10-1 to Figure 10-3) |
| Protocol for cell culture | (Appendix 10-1) |
| 10.2. In vitro spermatogenesis from primary spermatocytes |
(Figure 10-4 to Figure 10-5) |
| 10.3. Single cell labeling | (Figure 10-6 to Figure 10-8) |
| 10.4. Imaging of living embryo | (Figure 10-9 to Figure 10-10) |
| 10.5. Transplantation | (Figure 10-11 to Figure 10-14) |
| 10.6. Nuclear transplantation | (Figure 10-15 to Figure 10-16) |
| 10.7. Mutagenesis | (Figure 10-17 and Table 10-1 to Table 10-3) |
| Protocol for mutagenesis | (Appendix 10-2) |
| 10.8. TILLING (gene knockout) | (Figure 10-18
to Figure 10-19 and Table 10-4) |
| 10.9. Cell trace experiment with a caged fluorescent dye during medaka gastrulation |
(Figure 10-20 to Figure 10-23) |