Short villi on the egg envelope sometimes obstruct a clear view of the embryo. This movie shows how to remove these short villi.
1. Put fine sandpaper (P1200) in a Petri dish.
2. Put eggs on the sandpaper. (The attaching filament should be removed before this step. See M3-3a, b, c, and d.)
3. Rub eggs with a finger for a minute. If the villi are not sufficiently removed, continue to rub the eggs.
4. Transfer the eggs to a new Petri dish for culture or observation.