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Figure 3-6.
Detection of the Choriogenins in the ascites fluid obtained from the estrogen-fed male fish and SDS-PAGE pattern of purified choriogenins from the ascites fluid. (A) accumulation of the ascites fluid in the estrogen-fed male, a, normal male; b, estrogen-treated male. (B) native PAGE pattern of ascites fluid (a) and immunoblotting detection of Choriogenins with anti-egg envelope antibody (b). (C) SDS-PAGE pattern of isolated egg envelope (a), purified Choriogenin L (b), and Choriogenin H (c). (Reproduced from the Murata et al., 1993, with permission of Cambridge University Press.)