¹ The powder eggs contain about 250,000 eggs per gram and have approximately 0.62 g/cc in gravity.
² Salt concentration is not critical. About 2–3% salt concentration is acceptable for hatching. 3% salt is almost isotonic to seawater.
³ You can use common salt for cooking, artificial sea salt mix (commercial item), and NaCl for laboratory use.
⁴ You can use tap water (it is not necessary to use DW or any other purified water).
*; For toxicological studies, refer Chapter 8 about cautions in feedings or foods.