Brine shrimp preparation (separation and washing step). The hatched brine shrimp suspension contains unhatched eggs and eggshells and also a high concentration of salt. Separate and wash brine shrimp larva before feeding to medaka, especially small medaka larva. In Step 9, you must leave the runoff in Figure 2-25. Then decant larva layer into filter to wash nauplii (Step 10). Resuspend larvae with water and fill to near the bottle top, and then wait for 5–10 minutes to separate again (Step 11). If you find floating eggshells (Step 12), decant slightly to eliminate the eggshells. Decant nauplii layer to the filter again and stop decantation when you find sunk unhatched eggs (lower panels).